Table 1: Sense (S) and antisense (A) sequences of the primers used in the real-time PCR of target and housekeeping genes.

Gene Primer sequence (5′ to 3′) GeneBank accession number Amplicon size (base pairs) ALP S AACCCAGACACAAGCATTCC X13409 151 A GAGAGCGAAGGGTCAGTCAG BSP S GAAAATGGAGACGGCGATAG L20232 141 A ACCCGAGAGTGTGGAAAGTG CD44 S CTTCCATCTTGACCCGTTGT XM_283773 175 A ACAGTGCTCCTGTCCCTGAT Coll-I S AGAGCATGACCGATGGATTC NM_007742 177 A CCTTCTTGAGGTTGCCAGTC Dlx2 S AGTTCGTCTCCGGTCAACAA NM_010054.1 125 A GCCGCCAGCTGGAAACTGGA Hes1 S CTGCAGCGGGCGCAGATGAC NM_008235.2 114 A ACACGTGGACAGGAAGCGGG IGF-I S GCTCTTCAGTTCGTGTGTGG U75390 142 A ACATCTCCAGCCTCCTCAGA IL-6 S CCGGGAGCAGTGTGAGCTTA NM_031168 171 A TAGATGCGTTTGTAGGCGGTC OPG S AGACCATGAGGTTCCTGCAC U94331 131 A AAACAGCCCAGTGACCATTC Osx S ACTGGCTAGGTGGTGGTCAG NM_007419 135 A GGTAGGGAGCTGGGTTAAGG RANKL S GGCCACAGCGCTTCTCAG AF019048 141 A TGACTTTATGGGAACCCGAT Smad1 S ATGCCAGCTGACACACCCCC NM_008539.3 112 A TTTCAGCGGGCAGTGGAGGC Smad5 S GGAGTTTGCTCAGCTTCTGG NM_008541.2 134 A TGGTGACGTCCTGTCGGTGGT 18S rRNA S GTAACCCGTTGAACCCCATT X00686 151 A CCATCCAATCGGTAGTAGCG GAPDH S ACCCAGAAGACTGTGGATGG XM_132897 171 A CACATTGGGGGTAGGAACAC