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International Journal of Biomaterials
Volume 2012 (2012), Article ID 262890, 6 pages
http://dx.doi.org/10.1155/2012/262890
Research Article

Exploring the Mechanism of Microarteriogenesis in Porous Silk Fibroin Film

1School of Textile and Clothing Engineering, Soochow University, Suzhou 215021, China
2Faculty of Textile Science and Technology, Shinshu University, Ueda 386-8567, Japan

Received 8 May 2012; Revised 19 October 2012; Accepted 22 October 2012

Academic Editor: Rosalind Labow

Copyright © 2012 Lun Bai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose. Based on the experiment of the microarteriogenesis that is associated with angiogenesis during tissue repair process in porous silk fibroin films (PSFFs), we investigate the characteristics of micro-arteriogenesis and explore its mechanism. Methods. After the porous silk fibroin materials are implanted into the back hypodermal tissue of SD rats, the arteriole development and the morphogenesis of smooth muscle cell are histologically monitored and the micro-arteriogenesis is quantitatively analyzed. Results. 10 days after implantation, the arteriole density reaches the highest level in the junction of silk fibroin materials with tissues. Three weeks later, the arteriolar density in the materials reaches the maximum, and the arterioles in the junction of materials with tissues appear to be in a mature and upgrading state. Modeling of Microarteriogenesis. The arterioles in materials are generated after capillary angiogenesis. It is inferred that arteriolar development does not start until the network of the capillaries is formed. At first, the arterioles grow in the conjunct area of precapillaries with arterioles. Then with the extension of the arterioles, the upgrade of arterioles in connecting area is observed at a later stage. Based on the observation, the conditions and the mechanism of microarterializations as well as the upgrade of arterioles are analyzed.