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International Journal of Biomaterials
Volume 2012 (2012), Article ID 305638, 9 pages
Research Article

The Effect of Laminin-1-Doped Nanoroughened Implant Surfaces: Gene Expression and Morphological Evaluation

1Department of Prosthodontics, Faculty of Odontology, Malmö University, 205 06 Malmö, Sweden
2Division of Periodontology, Department of Oral Diagnosis and Surgery, School of Dentistry, UNESP, São Paulo State University, 01049-010 Araraquara, SP, Brazil
3Department of Biomaterials and Biomimetics, New York University, New York, NY 10010, USA
4Department of Clinical Dentistry, Center for Clinical Dental Research, University of Bergen, 5020 Bergen, Norway
5Division of Applied Prosthodontics, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8102, Japan
6Private Practice, Kobe 658-0012, Japan

Received 17 August 2012; Accepted 13 October 2012

Academic Editor: Carlos Nelson Elias

Copyright © 2012 Humberto Osvaldo Schwartz-Filho et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aim. This study aimed to observe the morphological and molecular effect of laminin-1 doping to nanostructured implant surfaces in a rabbit model. Materials and Methods. Nanostructured implants were coated with laminin-1 (test; dilution, 100 μg/mL) and inserted into the rabbit tibiae. Noncoated implants were used as controls. After 2 weeks of healing, the implants were removed and subjected to morphological analysis using scanning electron microscopy (SEM) and gene expression analysis using the real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Results. SEM revealed bony tissue attachment for both control and test implants. Real-time RT-PCR analysis showed that the expression of osteoblast markers RUNX-2, osteocalcin, alkaline phosphatase, and collagen I was higher (1.62-fold, 1.53-fold, 1.97-fold, and 1.04-fold, resp.) for the implants modified by laminin-1 relative to the control. All osteoclast markers investigated in the study presented higher expression on the test implants than controls as follows: tartrate-resistant acid phosphatase (1.67-fold), calcitonin receptor (1.35-fold), and ATPase (1.25-fold). The test implants demonstrated higher expression of inflammatory markers interleukin-10 (1.53-fold) and tumour necrosis factor-α (1.61-fold) relative to controls. Conclusion. The protein-doped surface showed higher gene expression of typical genes involved in the osseointegration cascade than the control surface.