The Effect of Laminin-1-Doped Nanoroughened Implant Surfaces: Gene Expression and Morphological Evaluation

Table 1

Primers used and specific parameters of the real-time PCR.

Gene	Primer sequence	Tm	Amplicon size (bp)	Primer source
ALP	S TGGACCTCGTGGACATCTG	75	80	Oryctolagus cuniculus
	A CAGGAGTTCAGTGCGGTTC
ATPase	S CCTGGCTATTGGCTGTTACG	77.7	98	Oryctolagus cuniculus
	A GCTGGTAGAAGGACACTCTTG
Calcitonin receptor	S CGTTCACTCCTGAAAACTACA	72.6	128	Oryctolagus cuniculus
	A GCAACCAAGACTAATGAAACA
Collagen I	S GGAAACGATGGTGCTACTGG	80.4	83	Oryctolagus cuniculus
	A CCGACAGCTCCAGGGAAG
IGF-1	S CCGACATGCCCAAGACTCA	70.3	81	Oryctolagus cuniculus
	A TACTTCCTTTCCTTCTCCTCTGA
IL-6	S GAGGAAAGAGATGTGTGACCAT	73.5	104	Oryctolagus cuniculus
	A AGCATCCGTCTTCTTCTATCAG
IL-10	S CCGACTGAGGCTTCCATTCC	73.3	75	Oryctolagus cuniculus
	A CAGAGGGTAAGAGGGAGCT
Osteocalcin	S GCTCAHCCTTCGTGTCCAAG	77.8	70	Oryctolagus cuniculus
	A CCGTCGATCAGTTGGCGC
Runx2	S GCAGTTCCCAAGCATTTCATC	72.8	81	Oryctolagus cuniculus
	A GTGTAAGTAAAGGTGGCTGGATA
TNF-α	S CTCACTACTCCCAGGTTCTCT	78.2	122	Oryctolagus cuniculus
	A TTGATGGCAGAGAGGAGGTT
TRAP	S GCTACCTCCGCTTCCACTA	78.5	129	Oryctolagus cuniculus
	A GCAGCCTGGTCTTGAAGAG
β-actin	S CACCCTGATGCTCAAGTACC	76.4	96	Oryctolagus cuniculus
	A CGCAGCTCGTTGTAGAAGG