BSA Nanoparticles for siRNA Delivery: Coating Effects on Nanoparticle Properties, Plasma Protein Adsorption, and In Vitro siRNA Delivery

Table 1

Selected characteristics of prepared NPs: (i) efficiency of PLL and PEG-PLL coating on BSA NPs, (ii) 𝜁-potential, and (iii) average size (average ± standard deviation, 𝑛≥5). Note that the NPs from 4.2 kDa PLL-PEG coating gave two size populations with relative ratios of 14% and 86%.

	Coated amount (mg conjugate/mg BSA)	𝜁-Potential  (mV)	Diameter  (nm)
Uncoated	—	−10.1±0.4	255±30
4.2 kDa PLL	0.12±0.03	10.1±0.5	350±90
24 kDa PLL	0.10±0.02	20.4±0.5	310±30
4.2 kDa PEG-PLL	0.016±0.006	1.8±0.3	220±26(14%)
			880±100(86%)
24 kDa PEG-PLL	0.007±0.003	7.8±0.9	845±140