Optimization of Human Corneal Endothelial Cells for Culture: The Removal of Corneal Stromal Fibroblast Contamination Using Magnetic Cell Separation
Figure 3
Following MACS of experimentally mixed human corneal endothelial cells and corneal stromal fibroblast using antifibroblast magnetic microbeads, cell fractions were subcultured as (a) the antifibroblast magnetic microbeads “labeled” fraction and (b) unlabeled “flow-through” fraction, 3 days after sorting. (Scale bar = 100 μm.) (c) A frequency histogram depicting the circularity of randomly selected cells in the “labeled” and the “flow-through” fractions. At least 100 cells from each fraction were counted.
