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International Journal of Biomaterials
Volume 2012 (2012), Article ID 632698, 9 pages
Research Article

Antifungal Activity of Chitosan Nanoparticles and Correlation with Their Physical Properties

1Drug Delivery and Novel Targeting Research Group, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur Campus, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
2Novel Antibiotic Research Group, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur Campus, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia

Received 8 February 2012; Revised 11 May 2012; Accepted 11 May 2012

Academic Editor: Thomas J. Webster

Copyright © 2012 Ling Yien Ing et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The need of natural antimicrobials is paramount to avoid harmful synthetic chemicals. The study aimed to determine the antifungal activity of natural compound chitosan and its nanoparticles forms against Candida albicans, Fusarium solani and Aspergillus niger. Chitosan nanoparticles were prepared from low (LMW), high molecular weight (HMW) chitosan and its derivative, trimethyl chitosan (TMC). Particle size was increased when chitosan/TMC concentration was increased from 1 to 3 mg/mL. Their zeta potential ranged from +22 to +55 mV. Chitosan nanoparticles prepared from different concentrations of LMW and HMW were also found to serve a better inhibitory activity against C. albicans ( M I C L M W = 0 . 2 5 –0.86 mg/mL and M I C H M W = 0 . 6 –1.0 mg/mL) and F. solani ( M I C L M W = 0 . 8 6 –1.2 mg/mL and M I C H M W = 0 . 5 –1.2 mg/mL) compared to the solution form ( M I C = 3  mg/mL for both MWs and species). This inhibitory effect was also influenced by particle size and zeta potential of chitosan nanoparticles. Besides, Aspergillus niger was found to be resistant to chitosan nanoparticles except for nanoparticles prepared from higher concentrations of HMW. Antifungal activity of nanoparticles prepared from TMC was negligible. The parent compound therefore could be formulated and applied as a natural antifungal agent into nanoparticles form to enhance its antifungal activity.