Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs

<table>Viability of MSCs on day 0 and 4 after encapsulation with and without cryopreservation: MSCs were stained with calcein AM for live (green) and ethidium homodimer for dead (red). On the day of microencapsulation ovine A MSCs stained for (a) live, (b) dead, and (c) overlay. And on day 4 following microencapsulation (e) ovine A MSCs stained for live, (f) dead, and (g) overlay. (d) Graphical representation of counts of 90 images. Cryopreserved ovine A encapsulated MSCs on day of thaw (i) live, (j) dead, and (k) overlay. Day 4 postthaw ovine A MSCs stained for (l) live, (m) dead, and (n) overlay. (h) Graphical representation of counts of 90 images (averaging 87,000 cells per group). (*<svg height="11.375" id="M19" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 75.025002 11.375" width="75.025002" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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International Journal of Biomaterials

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Figure 2

Figure 2: Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs 