865291.fig.004a
(a)
865291.fig.004b
(b)
865291.fig.004c
(c)
Figure 4: PA2-E12 cultured on tPS for 14 days in OM alone (a), in OM added with 30 μM Sr2+ (b), and without in the presence of gel-enriched with 3 mM Sr2+ (c). Cells fixed and stained for ALP. Observation in brightfield microscopy (10x objective): ALP+ cells intensity in red, ALP− cells in yellow, and HA deposits in black (green arrows).