Osteodifferentiation of Human Preadipocytes Induced by Strontium Released from Hydrogels

Figure 4

PA2-E12 cultured on tPS for 14 days in OM alone (a), in OM added with 30 μM Sr²⁺ (b), and without in the presence of gel-enriched with 3 mM Sr²⁺ (c). Cells fixed and stained for ALP. Observation in brightfield microscopy (10x objective): ALP+ cells intensity in red, ALP− cells in yellow, and HA deposits in black (green arrows).

(a)

(b)

(c)