Osteodifferentiation of Human Preadipocytes Induced by Strontium Released from Hydrogels

Table 1

Release and accumulation of Sr²⁺ in the culture medium from transwells with CMCA enriched with Sr²⁺.

Days of culture	[Sr^2+] accumulated in the culture medium in presence of CMCA enriched with 3 mM Sr^2+	[Sr^2+] accumulated in the culture medium in presence of CMCA enriched with 300 μM Sr^2+	[Sr^2+] accumulated in the culture medium in presence of CMCA enriched with 30 μM Sr^2+	[Sr^2+] accumulated in the culture medium in presence of CMCA enriched with 3 μM Sr^2+	Replacement of the old OM with fresh OM
1	1095 ± 33 mM	110 ± 3 𝝁 M	𝟏𝟐±𝟎.𝟒𝝁𝐌	<3 𝝁 M
2	1769 ± 68 mM	172 ± 5 μM	19 ± 0.5 μM	<3 μM
3	2185 ± 76 mM	220 ± 6 μM	20 ± 0.5 μM	<3 μM	X
4	256 ± 8 μM	24 ± 0.7 μM	2.6 ± 0.1 μM	<3 μM
5	413 ± 8 μM	43 ± 1.1 μM	4.2 ± 0.1 μM	<3 μM
6	510 ± 14 μM	50 ± 1.6 μM	5.1 ± 0.2 μM	<3 μM
7	570 ± 16 𝝁 M	59 ± 1.5 𝝁 M	𝟔.𝟎±𝟎.𝟐𝝁𝐌	<3 𝝁 M	X
8	37 ± 1 μM	3.6 ± 0.1 μM	<3 μM	<3 μM
9	59 ± 2 μM	6.0 ± 0.2 μM	<3 μM	<3 μM
10	73 ± 2 μM	7.2 ± 0.2 μM	<3 μM	<3 μM	X
11	9 ± 0.3 μM	<3 μM	<3 μM	<3 μM
12	14 ± 0.4 μM	<3 μM	<3 μM	<3 μM
13	17 ± 0.6 μM	<3 μM	<3 μM	<3 μM
14	19 ± 0.6 𝝁 M	<3 𝝁 M	<3 𝝁 M	<3 𝝁 M	X
17	<3 μM	<3 μM	<3 μM	<3 μM	X
21	<3 μM	<3 μM	<3 μM	<3 μM	X
28	<3 𝝁 M	<3 𝝁 M	<3 𝝁 M	<3 𝝁 M	X
42	<3 𝝁 M	<3 𝝁 M	<3 𝝁 M	<3 𝝁 M	X

Values expressed as means ± SD of Sr²⁺ at times for quantitative ALP and HA analysis are indicated in bold.