Development of Chitosan Nanoparticles as a Stable Drug Delivery System for Protein/siRNA
Table 1
Effects of DS : CS weight ratios on the physical characteristics of unloaded (top) and BSA loaded (below) CS NPs, . CS solution used for BSA loaded CS NPs was 0.1% w/v. BSA concentration used was 1 mg/mL.
(a)
DS (% w/v)
CS (% w/v)
DS : CS weight ratio
pH of nanoparticle dispersion
Particle size, nm ± SD
PDI ± SD
Surface charge, mV ± SD
0.05
0.10
0.5 : 1
3.84
*
*
*
0.10
0.10
1 : 1
3.79
*
0.15
0.10
1.5 : 1
3.80
*
0.20
0.10
2 : 1
3.81
*
0.25
0.10
2.5 : 1
3.82
*
*
(b)
DS (% w/v)
CS (% w/v)
DS : CS weight ratio
Particle size, nm ± SD
PDI ± SD
Surface charge, mV ± SD
EE, % ± SD
0.05
0.10
0.5 : 1
*
*
*
0.10
0.10
1 : 1
*
*
0.15
0.10
1.5 : 1
*
0.20
0.10
2 : 1
*
*
The mean difference is significant at the 0.05 level.
