Development of Chitosan Nanoparticles as a Stable Drug Delivery System for Protein/siRNA

Table 2

Effects of DS : CS weight ratios on the physical characteristics of siRNA loaded CS NPs, 𝑛=3. CS solution used for BSA loaded CS NPs was 0.1% w/v. siRNA concentration used was (15 µg/µL).

DS (% w/v)	CS (% w/v)	DS : CS weight ratio	Particle size, nm ± SD	PDI ± SD	Surface charge, mV ± SD	EE, % ± SD
0.05	0.10	0.5 : 1	330±50 *	0.45±0.01	63.6±5.9	94±3
0.10	0.10	1 : 1	540±30 *	0.50±0.02	53.0±4.0	95±4
0.15	0.10	1.5 : 1	776±40 *	0.51±0.01	20.1±5.2 *	92±2
0.20	0.10	2 : 1	900±60 *	0.60±0.04	6.0±4.0 *	90±2

∗The mean difference is significant at the 0.05 level.