Table 2: Effects of DS : CS weight ratios on the physical characteristics of siRNA loaded CS NPs, . CS solution used for BSA loaded CS NPs was 0.1% w/v. siRNA concentration used was (15 µg/µL).

DS (% w/v)CS (% w/v)DS : CS weight ratioParticle size, nm ± SDPDI ± SDSurface charge, mV ± SDEE, % ± SD

0.050.100.5 : 1 *
0.100.101 : 1 *
0.150.101.5 : 1 * *
0.200.102 : 1 * *

The mean difference is significant at the 0.05 level.