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International Journal of Biomaterials
Volume 2013 (2013), Article ID 949460, 10 pages
http://dx.doi.org/10.1155/2013/949460
Research Article

Generation of Chimeric “ABS Nanohemostat” Complex and Comparing Its Histomorphological In Vivo Effects to the Traditional Ankaferd Hemostat in Controlled Experimental Partial Nephrectomy Model

1Department of Urology, Ankara Training and Research Hospital, Ulucanlar, 06340 Ankara, Turkey
2Department of Gastroenterology, Turkiye Yuksek Ihtisas Training and Research Hospital, Sihhiye, 06100 Ankara, Turkey
3Institute of Materials Science and Nanotechnology, National Nanotechnology Research Center (UNAM), Bilkent University, 06800 Ankara, Turkey
4Department of Pathology, Ankara Training and Research Hospital, Ulucanlar, 06340 Ankara, Turkey
5Department of Biochemistry, Fatih University Medical School, 06560 Ankara, Turkey
6Department of Hematology, Hacettepe University Medical School, Sihhiye, 06100 Ankara, Turkey

Received 30 October 2012; Revised 13 December 2012; Accepted 20 January 2013

Academic Editor: Thomas J. Webster

Copyright © 2013 Emre Huri et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose. Using the classical Ankaferd Blood Stopper (ABS) solution to create active hemostasis during partial nephrectomy (PN) may not be so effective due to insufficient contact surface between the ABS hemostatic liquid agent and the bleeding area. In order to broaden the contact surface, we generated a chimeric hemostatic agent, ABS nanohemostat, via combining a self-assembling peptide amphiphile molecule with the traditional Ankaferd hemostat. Materials and Methods. In order to generate ABS nanohemostat, a positively charged Peptide Amphiphile (PA) molecule was synthesized by using solid phase peptide synthesis. For animal experiments, 24 Wistar rats were divided into the following 4 groups: Group 1: control; Group 2: conventional PN with only 0.5 ml Ankaferd hemostat; Group 3: conventional PN with ABS + peptide gel; Group 4: conventional PN with only 0.5 ml peptide solution. Results. Mean warm ischemia times (WITs) were , , , and seconds in Group 1 to Group 4, respectively. Fibrosis was not different among the groups, while inflammation was detected to be significantly different in G3 and G4. Conclusions. ABS nanohemostat has comparable hemostatic efficacy to the traditional Ankaferd hemostat in the partial nephrectomy experimental model. Elucidation of the cellular and tissue effects of this chimeric compound may establish a catalytic spark and open new avenues for novel experimental and clinical studies in the battlefield of hemostasis.