Antifungal Activity of Bioactive Compounds Produced by the Endophytic Fungus <i>Paecilomyces sp</i>. (JN227071.1) against <i>Rhizoctonia solani</i>

Hawar, Sumaiya Naeema; Taha, Zainab K.; Hamied, Atyaf Saied; Al-Shmgani, Hanady S.; Sulaiman, Ghassan M.; Elsilk, Sobhy E.

doi:https://doi.org/10.1155/2023/2411555

International Journal of Biomaterials

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Abstract Introduction Materials and Methods Results and Discussion Conclusions Data Availability Conflicts of Interest References Copyright Related Articles

Research Article | Open Access

Volume 2023 | Article ID 2411555 | https://doi.org/10.1155/2023/2411555

Antifungal Activity of Bioactive Compounds Produced by the Endophytic Fungus Paecilomyces sp. (JN227071.1) against Rhizoctonia solani

Sumaiya Naeema Hawar,¹Zainab K. Taha,²Atyaf Saied Hamied,¹Hanady S. Al-Shmgani,¹Ghassan M. Sulaiman,³and Sobhy E. Elsilk⁴

Academic Editor: Luca Fiorillo

Received28 Sept 2022

Revised08 Nov 2022

Accepted10 Apr 2023

Published20 Apr 2023

Abstract

Biologically active natural compounds are molecules produced by plants or plant-related microbes, such as endophytes. Many of these metabolites have a wide range of antimicrobial activities and other pharmaceutical properties. This study aimed to evaluate (in vitro) the antifungal activities of the secondary metabolites obtained from Paecilomyces sp. against the pathogenic fungus Rhizoctonia solani. The endophytic fungus Paecilomyces was isolated from Moringa oleifera leaves and cultured on potato dextrose broth for the production of the fungal metabolites. The activity of Paecilomyces filtrate against the radial growth of Rhizoctonia solani was tested by mixing the filtrate with potato dextrose agar medium at concentrations of 15%, 30%, 45%, and 60%, for which the percentages of inhibition of the radial growth were 37.5, 50, 52.5, and 56.25%, respectively. The dual culture method was conducted on PDA medium to observe the antagonistic nature of the antibiotic impacts of Paecilomyces sp. towards the pathogenic fungus. The strength of the antagonistic impacts was manifested by a 76.25% inhibition rate, on a scale of 4 antagonistic levels. Ethyl acetate extract of Paecilomyces sp. was obtained by liquid-liquid partition of the broth containing the fungus. Gas chromatography-mass spectrometry (GC-MS) analysis identified the presence of important chemical components e.g., (E) 9, cis-13-Octadecenoic acid, methyl ester (48.607), 1-Heptacosanol, 1-Nonadecene, Cyclotetracosane (5.979), 1,2-Benzenedicarboxylic acid, butyl 2-methylpropyl ester, di-sec-butyl phthalate (3.829), 1-Nonadecene, n-Nonadecanol-1, Behenic alcohol (3.298), n-Heptadecanol-1, 1-hexadecanol, n-Pentadecanol (2.962), Dodecanoic acid (2.849), 2,3-Dihydroxypropyl ester, oleic acid, 9-Octadecenal, and (Z)-(2.730). These results suggest that secondary metabolites of the endophytic Paecilomyces possess antifungal properties and could potentially be utilized in various applications, such as environmental protection and medicine.

1. Introduction

Biologically active natural compounds are molecules that are produced either by plants or plant-related microorganisms, such as endophytes. Many of these metabolites have a wide range of antimicrobial activities and other pharmaceutical properties [1]. Endophytic fungi are microorganisms that are held within plant tissue without stimulating obvious disease symptoms [2]. They are an important source of many secondary metabolites that are biologically active [3], such as tannins, flavonoids, coumarins, alkaloids, phenolic, peptides, lactones, phenylpropanoids, terpenes, polyketides, and lignans [4]. Fungal endophytes are a natural source of new, physiologically active substances that are significant for medicine [5]. Anticancer and antimicrobial agents derived from the endophytes fungi (more than 70%) are biologically active natural compounds or their derivatives [6]. Biologically active compounds demonstrated significant impacts on immunological diseases, such as hypocholesterolaemia and diabetes, as well as issues related to oxidative stress. They are also helpful in enhancing crops and reducing the negative effects of abiotic stresses [7].

Paecilomyces is a widespread filamentous fungus that inhabits environments such as food products, soil, and decomposing plants. It includes various species that are both harmful and saprophytic [8], some species can infect humans [9], nematodes [10], and can grow on the rhizosphere of many plants [11]. The interaction between the plant and Paecilomyces may also improve plant health and provide protection against plant pathogens through different mechanisms [12]. Paecilomyces acts as a biological control agent and therefore has positive effects on crop growth [13]. The genus Pacecilomyces has numerous species which can produce different secondary metabolites; with a total of 148 active metabolites have been reported [14] that can be used for agrochemicals or drugs development. They possess diverse biological activities, including cytotoxic [15], fungicidal [16], insecticidal [17], herbicidal [18], bactericidal [19], nematicidal [20], and antitumour [21]. Endophytic fungi help in promote plant competence through several mechanisms of action. The modes of action include endophytes-pathogens interactions (direct mechanisms) such as the production of antibiotics [22] and lytic enzymes [23], even though enzymes may not be merely effective as antagonism agent, they may enhance antagonistic activities when combined with other mechanisms. Competition is a powerful mechanism used by endophytes in preventing pathogens from colonizing the host tissue [24] and enhanced plant defense mechanisms (indirect mechanism) endophytes increase the plant defense mechanism by production of secondary metabolites and enhanced resistance of plant host. An example of innate pathogen resistance that has been developed may be a specific or unspecified resistance [25].

Many crop species are susceptible to the pathogenic fungus Rhizoctonia solani. It is the causal organism of stem canker and black scurf in potatoes (Solanum tuberosum L.). Being the latter the most prevalent disease in the nation [26]. Disease caused by Rhizoctonia can result in marketable yield losses of up to 30% in potato and considerable losses in quality. While quantitative losses result from infections of stems, stolons, and roots, which affect tuber size and number, qualitative losses are mostly caused by the creation of malformed tubers and the growth of sclerotia on the tuber surface (known as black scurf) [27]. Thus, as potatoes that are one of the basic nutrition sources of the world population, the purpose of the current study is to assess the antifungal activity against Rhizoctonia solani of secondary metabolites obtained from Paecilomyces sp.

2. Materials and Methods

2.1. Endophytic Fungus

The endophytic fungus Paecilomyces sp. (JN227071.1) was previously isolated and identified by polymerase chain reaction technique (PCR) [28] from Moringa olifera leaves. The fungal isolate was cultivated on potato dextrose agar (PDA) and stored in refrigerator at 4°C, the culture was transferred out every two months in new PDA medium.

2.2. Fungi Testing Culture

The pathogenic fungus Rhizoctonia solani was obtained from the Department of plant protection, Agriculture College, University of Baghdad. The fungus was maintained on PDA slant and stored at 4°C, before use transferred to PDA plate for 5–7 days at 28°C.

2.3. Fermentation and Extraction

In order to obtain bioactive compounds from Paecilomyces sp., the fungus isolate was cultured in potato dextrose broth (PDB). Conical flask (250 ml) containing 100 ml of PDB were inoculated with three agar plugs (5 mm) from 7 day old fungal, incubated at 28°C, 120 rpm for 14 days. Fermentation broth and fungal biomass were separated using a-Whatman filter paper (no. 1), and the culture broth was extracted by ethyl acetate (1 : 1 v\v). The organic extracts were evaporated by oven at (50°C) [29].

2.4. Antifungal Activity Assay on Potato Slices

Slices of potato tubers were used to investigate the bioactive chemicals produced by Paecilomyces Sp.; specifically for their ability to protect against Rhizoctonia solani, according to [30] with modifications. To sterilize the surface of potato tubers, sodium hypochlorite (2%), and (70%) ethanol alcohol were used; they were then washed three times with sterile distilled water (10 minutes each) and cut into slices under sterilized conditions. Potato slices were flooded in the fungal filtration medium or the PDB medium (control solution) for one hour in sterile dishes. The potato slices were then removed from the immersion solution, blotted with sterile filter paper to remove excess solution, and air-dried. They were then put in sterile dishes, while some slices were inoculate with a disk (5 mm) of pathogenic fungi and incubated at 28°C for 7 days. Each treatment was performed with three replicates.

2.5. Effects of Paecilomyces Filtrate on Rhizoctonia solani Growth

The growth of the pathogenic fungus Rhizoctonia solani was investigated to assess the effects of Paecilomyces filtrate. Fungal filtrate was added after sterilize with Millipore filter (0.22 μm) to the growth medium to obtain concentration of 15%, 30, 45, and 60%. Plates without fungal filtrate served as control. The mixture was mixed well and then poured in petri dishes until hardened. Rhizoctonia solani 5 mm disks (7 days old) were placed in the middle of the petri dishes and incubated at 28°C for 7 days. The growth inhibition percentage was calculated according to [31].

2.6. Test of Antagonistic Activity

The dual culture-plate antagonism assay was used to assess the influence of the endophytic fungus Paecilomyces sp. against R. solani [31]. The fungi were grown for seven days at 28°C on PDA medium in PDA Petri dishes. 5-mm plugs of Paecilomyces sp. and R. solani were co-cultured and incubated at 28 2°C. The plugs were positioned on either side of the Petri dish’s center with 4 cm distance from one another. R. solani was inoculated as control. The control’s colony size gradually approached that of the plate. All control and test plates were run in triplicate. The following parameters were used to assess pathogen growth inhibition: the percentage of R. solani radial growth inhibition at [100 × (R1 − R2)/R1], where R1 and R2 are the radial growth values of fungi in the control and tested plates, respectively [32]; (ii) the mode of inhibition on a scale of 0 to 4, with 0 denoting no growth inhibition, 1 denoting a growth inhibition of 1 to 25 percent, and 2 denoting a growth inhibition of 26 to 50 percent [33].

2.7. Gas Chromatography-Mass Spectrometry (GC/MS) Analysis

GC-MS analysis of ethyl acetate extracts of Paecilomyces sp. was performed using a Clarus 500/580 Perkin Elmer GC machine (Connecticut, USA), equipped with an AOC-20i auto-sampler and Elite-1 fused silica capillary column (100 percent methyl polysiloxane, 30 m, 0.25 mm, and 0.25 m), which was used to perform the extraction. As a carrier gas, helium (99.99 percent) was employed at a constant flow rate of 1 ml/min. Samples of 0.5 l were injected with a ratio (1 : 10), while the injector temperature was 280°C. The oven temperature was set to automatically rise from 110°C to 200°C and then at a rate of 5°C/min up to 280°C (10 min). With a runtime of 36 minutes, mass scans were performed in the range of 40–450 Da at electron energy of 70 eV (0.5 sec scan interval). Using the National Institute of Standards and Technology’s (NIST) database, the mass spectrum of the GC-MS was analysed. The spectrum of the components kept in the NIST collection was compared to the mass spectrum of the unidentified components [34].

2.8. Statistical Analysis

The SPSS software (version 23) was used to assess significant statistical analysis. All the experiments were carried out in triplicates. The one-way ANOVA and the Duncan’s test were used to assess whether there were any significant difference between the means.

3. Results and Discussion

3.1. The Protective Effects of Pacilomyces sp. Filtrate on the Growth of Rhizoctonia solani on Potato Slices

The results showed that potato slices treated with Paecilomyces filtrate alone, whether treated with the pathogenic fungus or not, had no clear growth of R. solani. The cell-free filtrate inhibited R. solani growth on potato slices, recording 100% inhibition at the stock solution concentration (Figures 1(a) and 1(b)). As for the potato slices treated in the PDB medium, whether with or without treatment with the pathogenic fungi, the growth of the R. solani appeared clear on the treated slices (Figures 1(c) and 1(d)). This result agrees with that reported previously [30]. An earlier work [31, 35] showed that Paecilomyces lilacinus (pt361) cell-free filtrate inhibited the leaf spot caused by S. sclerotiorum at all tested concentrations. Many studies have shown that different strains of Paecilomyces sp. produced metabolites with antifungal activities, like varioloid A, B [36], paciloxocin A, M [37], monocillin VI, VII [38], aigilomycin B, C, D, LL-2640-1, and 1,2-epoxyaigilomycin D [39]. According to studies, the synthesis of chitinase, leucinotoxins, acetic acid, and protease by Paecilomyces lilacinus is the factor responsible for the antinematode activity [40].

(a)

(b)

(c)

(d)

3.2. Effects of the Filtrate on Rhizoctonia solani Growth

The results showed that Paecilomyces filtrate has an effect on the radial growth of the pathogenic R. solani, starting from the concentration of 15% to 60%, compared with the control, but at different levels (Figure 2, Table 1). The percentages of radial growth inhibition of pathogenic R. solani were 37.5%, 50%, 52.5%, and 56.25% at the concentrations of 15, 30, 45, and 60%, respectively. A previous report [35] found that the cell-free filtrate of P. lilacinus (pt361) caused strong inhibition of radial mycelial growth of S. sclerotium, ranging from 60.3 to 100%. Another study [31] found that the mycelial growth, germ tube elongation, and spore germination of Penicillium digitatum were completely inhibited by the culture filtrate of Paecilomyces lilacinum at the concentration of 64%. Diketopiperazine, terezine D secondary metabolites derived from Paecilomyces cinnamomeus showed biological activities against Sordaria fimicola by their effects on radical growth, causing a 50% reduction at 200 μg/disk [41]. Farinomalein, a maleimide-containing chemical, isolated from Paecilomyces farinosus showed strong actions, at 5 g/disk, against the plant Phytophthora sojae [42]. Leucinostatin is a complex antibiotic that was isolated from Paecilomyces lilacinus [43], of which the compounds leucinostatin A [41] and leucinostatin B [44] were later separated and showed antibacterial and antifungal activities [45]. Parcilomyces lilacinum antifungal properties may be attributed to the existence of bioactive metabolites, such as the leucinostatins known as paecilotoxins [46]. Leucinostatins have been shown in several studies to be highly effective against a variety of fungi and bacteria [35, 47, 48].

3.3. Test of Antagonistic Activity

Dual culture method on the solid medium was performed in the current study to observe the antagonistic nature of the endophytic fungus Paecilomyces sp. against the pathogenic fungus Rhizoctonia solani. The results listed in Figure 3 show a strong antagonistic impact against the pathogen, where the inhibition rate was 76.25% and on a scale of 4 to determine the level of antagonistic according to [33]. Researchers have indicated that the antagonistic interactions between fungi can be considered as biological control against pathogens [49]. The difference between endophytic fungi and pathogenic indicated the emergence of a specific condition; the endophytic fungus grew above the pathogenic fungus at their point of contact, causing a highly significant inhibition of growth. This case indicates that there has been a mycoparasitism behavior by the endophytic fungus towards the pathogenic one. Researchers [50, 51] have shown that the condition of mycoparasitism is due to the secretion of enzymes by the parasitic fungus, which leads to the decomposition of the walls, and eventually the death, of the partner fungus. It has been found that the cell-free filtrate of Paecilomyces lilacinus (pt361) caused a 65% inhibition of radial growth of Seclerotinia sclerotiorum, as demonstrated by using the dual culture test, with an inhibition zone of 5.9 mm [35]. Also, it has been reported that, without any physical contact, Paecilomyces lilacinum was able to inhibit Penicillium digitatum growth by 68.2% based on the dual culture test [31]. It was suggested that this inhibition points to the presence of fungistatic metabolites produced by Paecilomyces lilacinum grown on the medium. The production of volatile organic molecules, such as acids, alcohols, alkenes, aldehydes, esters, terpenes, ketones, benzenoids, and pyrazines, as well as the production of bioactive metabolites, enzymes, and toxins, can cause in antagonism relation among the fungi without physical contact. These substances significantly contribute to the antagonistic effects and the fungal recognition systems through chemical signaling [52, 53].

(a)

(b)

(c)

3.4. GCMS Analysis

The ethyl acetate extract of Paecilomyces sp. showed a bright yellow color. The composition of the ethyl acetate extracts analyzed by GC-MS is presented in Table 2 and Figure 4. The analysis of Paecilomyces extract revealed the presence of of (E) 9, cis-13-Octadecenoic acid, methyl ester (48.607); 1-Heptacosanol, 1-Nonadecene, cyclotetracosane (5.979); 1,2-Benzenedicarboxylic acid, butyl 2-methylpropyl ester, Di-sec-butyl phthalate (3.829); 1-Nonadecene,n-Nonadecanol-1, behenic alcohol (3.298); n-Heptadecanol-1,1-Hexadecanol, n-Pentadecanol (2.962); Dodecanoic acid (2.849); 2,3-dihydroxypropyl ester, Oleic Acid, 9-Octadecenal, (Z)-(2.730).

The major components of the ethyl acetate extract of Paecilomyces were 9, cis-13-Octadecenoic acid, methyl ester, 10-Octadecenoic acid, and methyl ester, which belongs to a group of oleic acid esters with cancer preventive, insectifuge, anti-inflammatory, choleretic, and anemiagenic effects [54]. Due to the presence of many phytoconstituents, including various octadecanoic acid methyl esters, the Achyranthes ferruginea plant demonstrated considerable antioxidant, cytotoxic, and free radical scavenging capabilities [55]. Hexadecenoic acid methyl ester had the highest antimicrobial effect against clinical pathogenic bacteria [56]. The fatty acid composition, such as pentadenoic acid (a saturated fatty acids), and showed the antibacterial and antifungal activities of fatty acid methyl esters from the white oak plant extract [57]. Dimethyl phthalate may cause membrane channel misopening and cell membrane deformation. Isolated from B. mcbrellneri, di (2-ethylhexyl) phthalate and di-n-butyl phthalate exhibit a broad-spectrum of antibacterial activities [58]. Gram-positive S. epidermidis and S. aureus and Gram-negative E. coli, P. aeruginosa, and Klebsiella pneumoniae are all susceptible to being inhibited by phthalates. Di (2-ethylhexyl) phthalate from Calotropis gigantean flowers has antibacterial effects on B. subtilis [59] and antifungal activities against Candida albicans [60]. The chemical di-n-butyl phthalate, obtained from a new marine Streptomyces species [61], greatly reduced the spore germination and mycelial growth of Colletotrichum fragariae. Di-n-butyl phthalate was reported to inhibit mycelium growth and spore germination of Colletotrichum musae, Gaeumannomyces graminis, and Colletotrichum gloeosporioides [62, 63]. Long-chain primary alcohols are present in the S. amplexicaulis leaf extract. A crude extract can be made from the leaves of this plant, which is a synthetic mixture of six chemicals (1-tridecanol, 1-pentadecanol, 1-heptadecanol, 1-nonadecanol, 1-eicosanol, and 1-tricosanol). Lower amounts of S. amplexicaulis leaves demonstrated antibacterial action [64]. Cyclotetracosane (hydrocarbon) possesses higher biological activities than the other fractions of Jatropha zeyheri [65].

4. Conclusions

Paecilomyces sp. is a type of endophytic fungi that plays an important role in biological control. GC-mass fungus extract results show different types of secondary metabolites that possess antifungal and antibacterial properties. Antagonistic interactions between fungi can be considered as biological control against pathogens. The use of biologically active secondary metabolites of endophytic fungi as a biological control against plant diseases is essential and will help mitigate the harmful side effects of the use of synthetic pesticides on plant growth and crop production. Thus, the production of pesticides from the secondary metabolites of endophytic fungi will go a long way towards improving food security.

Data Availability

All data in this study are included within the article.

Conflicts of Interest

The authors declare that they have no conflicts of interest.

References

E. Song, C. Zhang, B. Israelow et al., “Neuroinvasion of SARS-CoV-2 in human and mouse brain,” Journal of Experimental Medicine, vol. 218, no. 3, Article ID e20202135, 2021.
View at: Publisher Site | Google Scholar
S. Pál, R. Tóth, J. Nosanchuk, C. Vágvölgyi, T. Németh, and A. A. Gácser, “A Candida parapsilosis overexpression collection reveals genes required for pathogenesis,” Journal of Fungi, vol. 7, no. 2, p. 97, 2021.
View at: Publisher Site | Google Scholar
N. Moghaddam, C. Dabard, M. Dufour et al., “Surface modification of CdE (E: S, Se, and Te) nanoplatelets to reach thicker nanoplatelets and homostructures with confinement-induced intraparticle type I energy level alignment,” Journal of the American Chemical Society, vol. 143, no. 4, pp. 1863–1872, 2021.
View at: Publisher Site | Google Scholar
K. P. Mulla, C. Dandu, V. Thirumalanadhuni, S. Shaik, I. A. Shaik, and Y. Nimmanapalli, “Phytochemical, elemental, physico-chemical, HPTLC and anticancer investigations of Ceropegia spiralis Wight. Tuber extracts,” The Pharma Innovation, vol. 10, no. 3, pp. 95–105, 2021.
View at: Publisher Site | Google Scholar
D. J. Newman and G. M. Cragg, “Natural products as sources of new drugs from 1981 to 2014,” Journal of Natural Products, vol. 79, no. 3, pp. 629–661, 2016.
View at: Publisher Site | Google Scholar
D. J. Newman and G. M. Cragg, “Natural products as sources of new drugs over the nearly four decades from 01/1981 to 09/2019,” Journal of Natural Products, vol. 83, no. 3, pp. 770–803, 2020.
View at: Publisher Site | Google Scholar
A. L. Khan, J. Hussain, A. Al-Rawahi et al., “Endophytic fungi: resource for gibberellins and crop abiotic stress resistance,” Critical Reviews in Biotechnology, vol. 35, no. 1, pp. 62–74, 2015.
View at: Publisher Site | Google Scholar
T. Gumus, A. S. Demirci, O. Sagdic, and M. Arici, “Inhibition of heat resistant molds: Aspergillus fumigatus and Paecilomyces variotii by some plant essential oils,” Food Science and Biotechnology, vol. 19, no. 5, pp. 1241–1244, 2010.
View at: Publisher Site | Google Scholar
J. Luangsa-ard, J. Houbraken, T. van Doorn et al., “Purpureocillium, a new genus for the medically important Paecilomyces lilacinus,” FEMS Microbiology Letters, vol. 321, no. 2, pp. 141–149, 2011.
View at: Publisher Site | Google Scholar
D. López-Lima, G. Carrión, and A. E. Núñez-Sánchez, “Isolation of fungi associated with Criconemoides sp. and their potential use in the biological control of ectoparasitic and semiendoparasitic nematodes in sugar cane,” Australian Journal of Crop Science, vol. 8, pp. 389–396, 2014.
View at: Google Scholar
P. Kilama, T. Dubois, D. Coyne, B. Niere, C. S. Gold, and E. Adipala, “Antagonism of Paecilomyces spp. isolated from banana (Musa spp.) roots and rhizosphere against Radopholus similis,” Nematropica, vol. 37, pp. 215–226, 2007.
View at: Google Scholar
C. Malhadas, R. Malheiro, J. A. Pereira, P. G. de Pinho, P. Baptista, and P. Baptista, “Antimicrobial activity of endophytic fungi from olive tree leaves,” World Journal of Microbiology and Biotechnology, vol. 33, no. 3, p. 46, 2017.
View at: Publisher Site | Google Scholar
R. Nesha and Z. A. Siddiqui, “Effects of Paecilomyces lilacinus and Aspergillus niger alone and in combination on the growth, chlorophyll contents and soft rot disease complex of carrot,” Scientia Horticulturae, vol. 218, pp. 258–264, 2017.
View at: Publisher Site | Google Scholar
X. Q. Li, K. Xu, X. M. Liu, and P. Zhang, “A systematic review on secondary metabolites of Paecilomyces species: chemical diversity and biological activity,” Planta Medica, vol. 86, Article ID 805, pp. 805–821, 2020.
View at: Publisher Site | Google Scholar
K. S. Nam, Y. S. Jo, Y. H. Kim, J. W. Hyun, and H. W. Kim, “Cytotoxic activities of ace-toxyscirpenediol and ergosterol peroxide from Paecilomyces tenuipes,” Life Sciences, vol. 69, no. 2, pp. 229–237, 2001.
View at: Publisher Site | Google Scholar
J. Pia¸tkowski, U. Krzyzewska, and U. Nawrot, “Antifungal activity of enthomopathogenic species of the genus Paecilomyces,” Mikologia Lekarska, vol. 10, no. 93–99, 2003.
View at: Google Scholar
X. B. Song, L. H. Zhang, A. T. Peng, B. P. Cheng, and J. F. Ling, “First report of Paecilomyces variotii isolated from citrus psyllid (Diaphorina citri), the vector of huanglongbing of citrus, in China,” Plant Disease, vol. 100, no. 12, p. 2526, 2016.
View at: Publisher Site | Google Scholar
M. Nakajima, K. Itoi, Y. Takamatsu et al., “Cornexistin: a new fungal metabolite with herbicidal activity,” Journal of Antibiotics, vol. 44, no. 10, pp. 1065–1072, 1991.
View at: Publisher Site | Google Scholar
J. J. Hyung, H. Kang, J. J. Joong, and K. Y. Soo, “Paecilomyces variotii extracts for preventing and treating infections caused by fish pathogenic microorganisms,” 2013051523. KR Patent, 2013.
View at: Google Scholar
M. Saha, S. Sarkar, B. Sarkar, B. K. Sharma, S. Bhattacharjee, and P. Tribedi, “Microbial siderophores and their potential applications: a review,” Environmental Science & Pollution Research, vol. 23, no. 5, pp. 3984–3999, 2016.
View at: Publisher Site | Google Scholar
L. He, W. Shi, X. Liu, X. Zhao, and Z. Zhang, “Anticancer action and mechanism of ergosterol peroxide from Paecilomyces cicadae fermentation broth,” International Journal of Molecular Sciences, vol. 19, no. 12, p. 3935, 2018.
View at: Publisher Site | Google Scholar
A. L. Gunatilaka, “Natural products from plant-associated microorganisms: distribution, structural diversity, bioactivity, and implications of their occurrence,” Journal of Natural Products, vol. 69, no. 3, pp. 509–526, 2006.
View at: Publisher Site | Google Scholar
F.-K. Gao, C.-C. Dai, and X.-Z. Liu, “Mechanisms of fungal endophytes in plant protection against pathogens,” African Journal of Microbiology Research, vol. 4, pp. 1346–1351, 2010.
View at: Google Scholar
A. Martinuz, A. Schouten, and R. Sikora, “Systemically induced resistance and microbial competitive exclusion: implications on biological control,” Phytopathology, pp. 260–266, 2012.
View at: Publisher Site | Google Scholar
L. Kiraly, B. Barna, and Z. Király, “Plant resistance to pathogen infection: forms and mechanisms of innate and acquired resistance,” Journal of Phytopathology, vol. 155, no. 7-8, pp. 385–396, 2007.
View at: Publisher Site | Google Scholar
M. S. Ali and T. K. Dey, “Pathological research on tuber crops in Bangladesh,” in Proceedings of the Workshop on Tubers Crops on Transfer of Technology of CDP Crops under Research Extension Linkage Programme, pp. 159–165, New York, NY, USA, August 1994.
View at: Google Scholar
G. J. Banville, D. E. Carling, and B. E. Otrysko, “Rhizoctonia disease on potato,” in Rhizoctonia Species. Taxonomy, Molecular Biology, Ecology, Pathology and Disease Control, B. Sneh, S. Jabaji-Hare, S. Neate, and G. Dijst, Eds., pp. 321–330, Kluwer Academic, Dordrecht, the Netherlands, 1996.
View at: Google Scholar
M. M. Gufran and N. Hawar, “Morphological and molecular characterization of endophytic fungi isolated from Moringa oleifera leaves in Iraq and chemical analysis of leaves extracts using GC-Mass,” Asian Journal of Water Environment and Pollution, vol. 20, no. 6, 2023.
View at: Google Scholar
R. N. Kharwar, A. L. Maurya, V. C. Verma, A. Kumar, S. K. Gond, and A. Mishra, “ADiversity and antimicrobial activity of endophytic fungi community isolated from medicinal plant Cinnamomum camphora,” Proceedings of the National Academy of Sciences, India - Section B: Biological Sciences, vol. 82, no. 4, pp. 557–565, 2012.
View at: Publisher Site | Google Scholar
S. Elkahoui, N. Djébali, N. Yaich et al., “Antifungal activity of volatile compounds-producing Pseudomonas P2 strain against Rhizoctonia solani,” World Journal of Microbiology and Biotechnology, vol. 31, no. 1, pp. 175–185, 2014.
View at: Publisher Site | Google Scholar
E. A. Elsherbiny, M. A. Taher, M. H. Abd El-Aziz, and S. Y. Mohamed, “Action mechanisms and biocontrol of Purpureocillium lilacinum against green mould caused by Penicillium digitatum in orange fruit,” Journal of Applied Microbiology, vol. 131, no. 3, pp. 1378–1390, 2021.
View at: Publisher Site | Google Scholar
D. J. Royse and S. M. Ries, “The influence of fungi isolated from peach twigs on the pathogenicity of Cytospora cincta,” Pyhtopathol, vol. 68, pp. 603–607, 1978.
View at: Google Scholar
S. Zivkovic, S. Stojanovic, Z. Ivanovic, V. Gavrilovic, T. Popovic, and J. Balaz, “Screening of antagonistic activities of microorganisms against Colletotrichium acutatum and Colletotrichum gleosporioides,” Archives of Biological Sciences, vol. 62, no. 3, pp. 611–623, 2010.
View at: Publisher Site | Google Scholar
H. S. Al-Shmgani, Z. H. M. Kadri, M. M. Al-Halbosiy, and Y. H. Dewir, “Phytochemical analysis, cytotoxicity and antioxidant activity of cuckoo pint (Arum maculatum) leaf extract,” Acta Biologica Szegediensis, vol. 63, no. 2, pp. 119–124, 2020.
View at: Publisher Site | Google Scholar
F. Yang, H. Abdelnabby, and Y. A. Xiao, “Mutant of the nematophagous fungus Paecilomyces lilacinus (Thom) is a novel biocontrol agent for Sclerotinia sclerotiorum,” Microbial Pathogenesis, vol. 89, pp. 169–176, 2015.
View at: Publisher Site | Google Scholar
P. Zhang, X. M. Li, J. N. Wang, and B. G. Wang, “Oxepine-containing diketopiperazine alkaloids from the algal-derived endophytic fungus Paecilomyces variotii EN-291,” Helvetica Chimica Acta, vol. 98, no. 6, pp. 800–804, 2015.
View at: Publisher Site | Google Scholar
L. X. Xu, P. Wu, H. H. Wei et al., “Four new β-resorcylic acid lactones from Paecilomyces sp. SC0924,” Tetrahedron Letters, vol. 54, no. 21, pp. 2648–2650, 2013.
View at: Publisher Site | Google Scholar
F. Qin, Y. Li, R. Lin et al., “Antibacterial radicicol analogues from Pochonia chlamydosporia and their biosynthetic gene cluster,” Journal of Agricultural and Food Chemistry, vol. 67, no. 26, pp. 7266–7273, 2019.
View at: Publisher Site | Google Scholar
L. X. Xu, J. H. Xue, Y. Zou, S. J. He, and X. Y. Wei, “Three new β-resorcylic acid lactones from Paecilomyces sp. SC0924,” Chinese Journal of Chemistry, vol. 30, no. 6, pp. 1273–1277, 2012.
View at: Publisher Site | Google Scholar
J. O. Park, J. R. Hargreaves, E. J. McConville, G. R. Stirling, E. L. Ghisalberti, and K. Sivasithamparam, “Production of leucinostatins and nematicidal activity of Australian isolates of Paecilomyces lilacinus (Thom) Samson,” Letters in Applied Microbiology, vol. 38, no. 4, pp. 271–276, 2004.
View at: Publisher Site | Google Scholar
M. Isaka, S. Palasarn, K. Kocharin, and N. L. Hywel-Jones, “Comparison of the bioactive secondary metabolites from the scale insect pathogens, anamorph Paecilomyces cinnamomeus, and teleomorph Torrubiella luteorostrata,” Journal of Antibiotics, vol. 60, no. 9, pp. 577–581, 2007b.
View at: Publisher Site | Google Scholar
S. P. Putri, H. Kinoshita, F. Ihara, Y. Igarashi, and T. Nihira, “Farinomalein, a maleimide-bearing compound from the entomopathogenic fungus Paecilomyces farinosus,” Journal of Natural Products, vol. 72, no. 8, pp. 1544–1546, 2009.
View at: Publisher Site | Google Scholar
T. Arai, Y. Mikami, K. Fukushima, T. Utsumi, and K. Yazawa, “A new antibiotic, leucinostatin, derived from Penicillium lilacinum,” Journal of Antibiotics, vol. 26, no. 3, pp. 157–161, 1973.
View at: Publisher Site | Google Scholar
Y. Wang, J. B. Gloer, J. A. Scott, D. Malloch, and A.-D. Terezines, “New amino acid-derived bioactive metabolites from the coprophilous fungus Sporormiella teretispora,” Journal of Natural Products, vol. 58, no. 1, pp. 93–99, 1995.
View at: Publisher Site | Google Scholar
K. Fukushima, T. Arai, Y. J. Mori, M. Tsuboi, and M. Suzuki, “Studies on peptide antibiotics, leucinostatins. II: the structures of leucinostatins A and B,” Journal of Antibiotics, vol. 36, no. 12, pp. 1613–1630, 1983.
View at: Publisher Site | Google Scholar
S. Kuwata, A. Nakanishi, T. Yamada, and T. Miyazawa, “Total synthesis of leucinostatin D,” Tetrahedron Letters, vol. 33, no. 46, pp. 6995–6998, 1992.
View at: Publisher Site | Google Scholar
L. Radios, M. Kajtar-Peredy, C. G. Casinovi et al., “Two novel peptide antibiotics with tertiay amine-oxide terminal group from Paecilomyces marquandii Isolation, structure and biological activity,” Journal of Antibiotics, vol. 40, no. 5, pp. 714–716, 1987.
View at: Publisher Site | Google Scholar
G. Wang, Z. Liu, R. Lin et al., “alBiosynthesis of antibiotic leucinostatins in bio-control fungus Purpureocillium lilacinum and their inhibition on Phytophthora revealed by genome mining,” PLoS Pathogens, vol. 12, no. 7, Article ID e1005685, 2016.
View at: Publisher Site | Google Scholar
P. Abirami and A. Rajendran, “Antagonism interaction of some soil fungi against Trichophyton rubrum,” World Journal of Science and Technology, vol. 1, no. 6, pp. 40–42, 2011.
View at: Google Scholar
N. T. Garoe, R. Cabrera, C. Andreea, T. T. Martin, and G. Cristina, “Survey of banana endophytic fungi isolated inartificial culture media from an applied view point,” J. Horticult, Forestry & Biotechnol., vol. 17, no. 2, pp. 22–25, 2013.
View at: Google Scholar
S. Gomathi and V. Ambikapathy, “Antagonistic activity of fungi against Pythium debaryanum(Hesse) isolates from chilli field soil,” Advances in Applied Science Research, vol. 2, no. 4, pp. 291–299, 2013.
View at: Google Scholar
C. R. Howell, “Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts,” Plant Disease, vol. 87, no. 1, pp. 4–10, 2003.
View at: Publisher Site | Google Scholar
E. A. Elsherbiny, B. H. Amin, B. Aleem, K. L. Kingsley, and J. W. Bennett, “Trichoderma volatile organic compounds as a biofumigation tool against late blight pathogen Phytophthora infestans in postharvest potato tubers,” Journal of Agricultural and Food Chemistry, vol. 68, no. 31, pp. 8163–8171, 2020.
View at: Publisher Site | Google Scholar
H. Sheeba, M. Syed Ali, and V. Anuradha, “Antagonistic activity of endophytic fungi from the stem of Ziziphus mauritiana and Hybanthus enneaspermus,” International Journal of Research and Analytical Reviews, vol. 6, no. 2, pp. 445–451, 2019.
View at: Google Scholar
A. S. M. A. Reza, M. A. Haque, J. Sarker et al., “Antiproliferative and antioxidant potentials of bioactive edible vegetable fraction of Achyranthes ferruginea Roxb. in cancer cell line,” Food Science and Nutrition, vol. 9, no. 7, pp. 3777–3805, 2021.
View at: Publisher Site | Google Scholar
M. T. Shaaban, M. F. Ghaly, and S. M. Fahmi, “Antibacterial activities of hexadecanoic acid methyl ester and green-synthesized silver nanoparticles against multidrug-resistant bacteria,” Journal of Basic Microbiology, vol. 61, no. 6, pp. 557–568, 2021.
View at: Publisher Site | Google Scholar
A. Sati, S. C. Sati, N. Sati, and O. P. Sati, “Chemical composition and antimicrobial activity of fatty acid methyl ester of Quercus leucotrichophora fruits,” Natural Product Research, vol. 31, no. 6, pp. 713–717, 2016.
View at: Publisher Site | Google Scholar
R. N. Roy, S. Laskar, and S. K. Sen, “Dibutyl phthalate, the bioactive compound produced by Streptomyces albidoflavus 321.2,” Microbiological Research, vol. 161, no. 2, pp. 121–126, 2006.
View at: Publisher Site | Google Scholar
M. R. Habib and M. R. Karim, “Antimicrobial and cytotoxic activity of di-(2-ethylhexyl) phthalate and anhydrosophoradiol-3-acetate isolated from Calotropis gigantea (linn.) flower,” Mycobiology, vol. 37, no. 1, pp. 31–36, 2009.
View at: Publisher Site | Google Scholar
S. M. Shanab, E. A. Shalaby, D. A. Lightfoot, and H. A. El-Shemy, “Allelopathic effects of water hyacinth [Eichhornia crassipes],” PLoS One, vol. 5, no. 10, Article ID e13200, 2010.
View at: Publisher Site | Google Scholar
X. Li, T. Jing, D. Zhou et al., “Biocontrol efficacy and possible mechanism of Streptomyces sp. H4 against postharvest anthracnose caused by Colletotrichum fragariae on strawberry fruit,” Postharvest Biology and Technology, vol. 175, pp. 111401–112021, 2021.
View at: Publisher Site | Google Scholar
G. Qi, H. Liu, Z. Li, L. Long, and M. Wang, “Active constituents of garlic bulb extract and its inhibition on Colletotrichum gloeosporioides,” Journal of Henan Agricultural University, vol. 54, pp. 59–63, 2020.
View at: Google Scholar
C. Liang, Y. Chen, C. Li et al., “Antifungal activity of extracts of Helicteres ngustifolia against ten plant pathogenic fungi,” Guihaia, vol. 40, pp. 715–726, 2020.
View at: Google Scholar
S. Chatterjee, A. Karmakar, S. A. Azmi, and A. Barik, “Antibacterial activity of long-chain primary alcohols from solena amplexicaulis leaves,” Proceedings of the Zoological Society, vol. 71, no. 4, pp. 313–319, 2017.
View at: Publisher Site | Google Scholar
M. Ni, O. S. Soyingbe, and T. J. Makhafola, “Antimicrobial, cytotoxicity, anticancer and antioxidant activities of Jatropha zeyheri Sond. roots (Euphorbiaceae),” Asian Pacific Journal of Tropical Biomedicine, vol. 9, no. 7, pp. 307–314, 2019.
View at: Google Scholar

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Copyright © 2023 Sumaiya Naeema Hawar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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