]>Alteration of Sarcoplasmic Reticulum <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msup><mml:mrow><mml:mtext>Ca</mml:mtext></mml:mrow><mml:mrow><mml:mtext>2</mml:mtext><mml:mtext>+</mml:mtext></mml:mrow></mml:msup></mml:mrow></mml:math> Release in Skeletal Muscle from Calpain 3-Deficient Mice : Figure 6
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Figure 6: Effect of caffeine on [Ca2+]i in isolated myotubes in the presence of Ca2+ channel blockers (Ni2+/Cd2+). Representative traces show the typical time course of the response to 20 mM caffeine observed in (a) wild type (+/+) myotube; (c) calpain 3-deficient (−/−) myotube, each treated with Ni2+/Cd2+. The duration of exposure to caffeine (open bars), or 50  𝜇 M Ni2+/100  𝜇 M Cd2+ (closed bars) is represented. Bar diagrams (b) and (d) summarize the peak amplitude of the [Ca2+]i response of myotubes to caffeine and CPA. The responses from the wild type myotubes (+/+; b) and calpain 3-deficient myotubes (−/−; d), are shown. Fifty  𝜇 M Ni2+/100  𝜇 M Cd2+ was added to the extracellular medium prior to the second application. The number of cells tested is given in brackets.