Evaluation of X-Inactivation Status and Cytogenetic Stability of Human Dermal Fibroblasts after Long-Term Culture

<table><i>FISH analysis of the number of total X-chromosomes as well as the number of inactive Xs.</i> The total number of X-chromosomes in HDF cells at passage 10 was detected by DNA FISH analysis of the centromeric region of X chromosome (green) and the number of inactive X chromosomes by <i>XIST</i> RNA FISH assay (red). Note that the double signals for <i>XIST </i>RNA are indicative of the two inactive Xs, which can be observed in cell nucleus with either quadruple-signals for X chromosome (A) or triple signals for X chromosome (B).  Cell nucleus was counterstained with DAPI (blue).</table>

International Journal of Cell Biology

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Figure 4

Figure 4: Evaluation of X-Inactivation Status and Cytogenetic Stability of Human Dermal Fibroblasts after Long-Term Culture 