Evaluation of X-Inactivation Status and Cytogenetic Stability of Human Dermal Fibroblasts after Long-Term Culture

Table 1

Quantitation of the percentage of HDF cells without, or with one or two punctate signal(s) for H3K27me3 staining in HDF no.1–8.

Cell line	Passage 1			Passage 5			Passage 10
	S0	S1	S2	S0	S1	S2	S0	S1	S2
HDF-1	34	166	0	44	156	0	47	137	26
HDF-2	28	172	0	38	162	0	37	131	32
HDF-3	40	160	0	48	152	0	52	111	37
HDF-4	26	174	0	31	169	0	80	120	0
HDF-5	22	178	0	32	168	0	62	138	0
HDF-6	42	158	0	44	156	0	64	136	0
HDF-7	39	161	0	51	149	0	74	126	0
HDF-8	24	176	0	41	159	0	67	116	17

S0, S1, and S2, respectively, represent zero, one, and two H3K27me3 signal(s) observed in each nucleus.