Review on Trypanosoma cruzi: Host Cell Interaction
Figure 6
(a, b) DTAF (sialic acid marker) labeled macrophages were incubated with trypomastigote forms of Trypanosoma cruzi for 1 hr at 37°C. Observation by confocal laser scanning microscope (CLSM): DIC image (a) and corresponding fluorescence (b) images. Observe the labeling of the membrane lining the parasitophorous vacuole containing a trypomastigote form (arrow); (c, d) macrophages labeled with PKH-26 (lipid marker) were allowed to interact with trypomastigote forms of T. cruzi for 1 hr at 37°C. Observation by CLSM: DIC (c) and corresponding fluorescence images. Observe labeling of the plasma membrane in the parasitophorous membrane containing a trypomastigote form (arrow). Bar: 5 m (after [18]).
