Review on Trypanosoma cruzi: Host Cell Interaction
Figure 7
Trypomastigote vacuole is enriched in AKT-PH-GFP that binds to posphatidylinositolbiphosphate (PtdIns(3,4)P2) and phosphatidylinositoltriphosphate (PtdIns(3,4,5)P3) that are products of PI3 Kinase (a–c) and also labeled to EEA1 and Rab 5 (d–f) and Lamp-1 (g–i). (a–c) rapid accumulation of PtdIns(3,4,5)P3/PtdIns (3,4)P2 at the T. cruzi invasion site. Fluorescence images of (a) CHO cells or (b) primary rat cardiomyocytes transiently expressing Akt-PH-GFP following incubation with infective T. cruzi trypomastigotes for 15 minutes. (c) T. cruzi invasion of CHO cells expressing Akt-PH-GF which fails to bind to PtdIns (3,4,5)P3/PtdIns(3,4)P2. (d)–(f): early association of T. cruzi with early endosomes is minimal and precedes lamp-1 acquisition. Immunofluorescence staining of extracellular T. cruzi (arrowheads) following infection of CHO cells transiently expressing Rab5-GFP (green). Host cell and parasite DNA is visualized with DAPI (blue). (g–i) lamp-1 association with the Akt-PH-GFP-enriched T. cruzi vacuole occurs after parasite entry. L6E9 myoblasts expressing Akt-PH-GFP (green) were infected with T. cruzi for 60 minutes and stained with anti-lamp-1 (red) and DAPI (blue). Bars = 5 m (after [28]).
