Inhibition by Anandamide of 6-Hydroxydopamine-Induced Cell Death in PC12 Cells
Figure 2
Anandamide-mediated prosurvival effect is cannabinoid and vanilloid receptor-independent. PC12 cells were treated with 25 M anandamide for 24 hours, with or without selective receptor antagonists, and then exposed to 100 M 6-OHDA for a further 24 hours. Effector caspase activity was measured by DEVDase assay in whole cell extracts. (a) Indicated concentrations of receptor antagonist SR141716A were added 1 hours prior to incubation with anandamide. Values represent the mean ± SEM of 3 independent determinations, (b) Indicated concentrations of receptor antagonist SR144528 were added 1 hour prior to incubation with anandamide. Values represent the mean ± SEM of 3 independent determinations, (c) The TRPV1 receptor antagonist SB366791 at 10 M was added 1 hour prior to incubation with anandamide. The data shown are average of two separate experiments ± range.
