Research Article

Receptor-Mediated Transcytosis of Leptin through Human Intestinal Cells In Vitro

Figure 1

Transcytosis of leptin through Caco-2/15 cell monolayer. (a) Intestinal caco 2/15 cells grow as a tight polarized monolayer displaying apical microvillosities (mv) and tight junctional complexes (J). (b) Upon preparation of the leptin-FITC complex, leptin is revealed by Western blot as a single band of 16 kDa using antibodies against leptin as well as against FITC. (c) Quantification of leptin by Western blot is linear up to 1 ng/mL. (d) Leptin-FITC (10  g/mL) added to the apical compartment of Caco-2/15 cell monolayer is transported to the basal compartment along with time and appears to be saturable. (e) Leptin intracellular content was raised significantly at the end of the experiment (60 minutes). Results are expressed as mean values s.e.m ( = 4 for each experiments).
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