Receptor-Mediated Transcytosis of Leptin through Human Intestinal Cells In Vitro
Figure 6
Inhibition of leptin endocytosis. Caco-2/15 cells were preincubated with the following inhibitors of endocytosis: Amiloride (Amil) (3 mM), chlorpromazine (Chlor) (6 g/mL), and nystatin (Nyst) (25 g/mL). Leptin was added to the apical medium for 30 minutes and intracellular leptin contents were measured. (a) A significant decrease was observed only with chlorpromazine treatment. (b) Specificity of chlorpromazine treatment was confirmed by measuring of intracellular leptin at shorter incubation times (2, 5, and 10 minutes). Mean values s.e.m ( = 4).
