Receptor-Mediated Transcytosis of Leptin through Human Intestinal Cells In Vitro
Figure 7
Involvement of the Golgi apparatus in leptin transcytosis. Caco-2 cells were exposed to leptin-FITC for 30 minutes. (a) By confocal microscopy, leptin (green fluorescence) and TGN42, a marker of the Golgi apparatus (red fluorescence), appear colocalized (yellow staining) in spots ( plane) or as crescent-shaped structures close to the nuclei ( plane). (b) Quantification of signal for leptin and TGN42 in view confirmed colocalization. (c) Inhibitors of Golgi trafficking, brefeldin A (Bref) (10 g/mL), and okadaic acid (Okad.a) (0.5 M) were added to the cell medium for 30 minutes prior adding leptin. Cellular content (c1) and concentrations of leptin in the basal medium (c2) were decreased by the two agents. Results are expressed as mean values s.e.m ( = 4).
