Identification and Characterization of Novel Perivascular Adventitial Cells in the Whole Mount Mesenteric Branch Artery Using Immunofluorescent Staining and Scanning Confocal Microscopy Imaging
Figure 1
Immunofluorescent confocal analysis of the whole mount rat MBA showing the CaSR and palladin expression. ANNIES and the nerve fibers strongly express (94 ± 6%) the CaSR protein (top panel—in red, alexa fluor—647). Fibroblast like cells with no processes, and cells with nerve fibers (ANNIES) showing palladin expression (80 ± 10%) in red (lower panel). Nuclei stained in green (sytox)—(100x), (g). Rat MBA stained for secondary antibody alone, tagged with alexa fluor-647, and nuclear stain sytox—(100x). (Representative of 3 experiments.)
