WIPI-1 Positive Autophagosome-Like Vesicles Entrap Pathogenic Staphylococcus aureus for Lysosomal Degradation

Figure 7

Apathogenic S. carnosus TM300 cells are not entrapped in GFP-WIPI-1 positive autophagosome-like vesicles. According to Figures 4–6, GFP-WIPI-1 U2OS cells were infected with mCherry-expressing S. carnosus TM300 in DMEM/FCS (a), DMEM (b), and EBSS (c) and analyzed (up to 2000 individual cells). Representative images (2 h, DMEM/FCS) are presented ((d), scale bars: 20 μm). The number of cells with GFP-WIPI-1 positive autophagosomal-like vesicles entrapping S. carnosus TM300 is presented (e) ± SD (𝑛=2, each in duplicates).