S-Glutathionylation of Protein Disulfide Isomerase Regulates Estrogen Receptor Stability and Function
Figure 3
PABA/NO abrogates PDI-ERα interaction. (a) MCF7 cell lysates (1 mg) were incubated with anti-ERα antibody or control IgG at 4°C overnight. (b) MCF7 cells were treated with DMSO or 20 μM PABA/NO for the indicated times. Cell lysates (300 μg) were incubated with anti-PDI antibody at room temperature for 4 h. The precipitated endogenous PDI was subjected to Western blot with anti-S-glutathionylation (P-SSG) and anti-PDI antibodies. (c) MCF7 cells were treated with DMSO or 20 μM PABA/NO for the indicated times. Endogenous ERα was precipitated with the anti-ERα antibody at 4°C overnight. The precipitates were subjected to Western blot with anti-ERα and anti-PDI antibodies.
