S-Glutathionylation of Protein Disulfide Isomerase Regulates Estrogen Receptor Stability and Function
Figure 4
PABA/NO leads to ERα proteasomal degradation. (a) MCF7 cells were treated with DMSO or 20 μM PABA/NO for the indicated times. Cell lysates (40 μg) were subjected to Western blot with the indicated antibodies. (b) MCF7 cells were treated with DMSO or 20 μM PABA/NO for the indicated times. (c) MCF7 cells were pretreated with proteasome inhibitors MG132 (1 μM) or PS341 (10 μM) for 2 h and then treated with 20 μM PABA/NO for 6 h. Cell lysates (40 μg) were subjected to Western blot with the indicated antibodies. Bar diagram showed the means and standard deviations determined from three independent experiments.
