PKC-Mediated ZYG1 Phosphorylation Induces Fusion of Myoblasts as well as of Dictyostelium Cells

Figure 1

Localization of GFP and GFP/ZYG1 fusion protein. GFP^CONT and GFP/ZYG1^OE cells are starved for 2 h, fixed with 4% paraformaldehyde for 20 min, and observed under a fluorescence microscope. GFP protein in GFP^CONT cells and GFP/ZYG1 fusion protein in GFP/ZYG1^OE cells are shown as the green color of GFP. GFP/ZYG1 fusion protein is preferentially localized at the periphery of vesicles (c, d; arrow) and at the phagocytic cups which are attached to form cell-cell contacts (f; arrows). On the other hand, in GFP^CONT cells, GFP protein is uniformly distributed in the cytoplasm (a, b; arrow), and its localization at the region of cell-cell contacts is not observed (e; arrow). This indicates that its localization is due to the presence of ZYG1, but not to GFP itself. Photographs of (b) and (c) are the magnified (a) and (d), respectively, and adjusted to get brighter images. Bars: 20 μm.