PKC-Mediated ZYG1 Phosphorylation Induces Fusion of Myoblasts as well as of Dictyostelium Cells
Figure 5
The phosphorylation of GFP/ZYG1 fusion protein by cPKC occurs at the region of cell fusion. Starved GFP/ZYG1OE cells were developed for 2 h in the presence of TPA (5 nM). The sample of GFP/ZYG1OE cells for the immunocytochemical detection was prepared as described in Figure 4. The same fields are shown as images of DIC (a), Rhodamine (b), GFP (c), and GFP and Rhodamine merged (d). Since it is evident by the DIC image that there is no boundary between the two cells (a; black arrow), cell fusion is occurring between two cells. Since the merged image shows yellow color (d; a white arrow), it is evident that GFP/ZYG1 fusion protein and proteins phosphorylated by cPKC are colocalized particularly at the region where cell fusion occurred. Bar: 25 μm.
