Partitioning and Exocytosis of Secretory Granules during Division of PC12 Cells
Figure 8
Distribution of SGs during cytokinesis. Single confocal images of immunostained PC12 cells in cytokinesis are shown as indicated. The main image (A) shows a merged presentation of microtubules and SGs; the single channels are shown in grayscale in (B) and (C), respectively. During cytokinesis, SGs were found to accumulate at the bases of the intercellular bridge ((A), arrowheads), but not in the midbody ((A) and (C)). ((D) and (D1)) PC12 cells in cytokinesis observed at the ultrastructural level. Shown in (D) are two PC12 daughter cells during cytokinesis which are connected by a midbody ((D), red dashed lines). The marked area (box in (D)) is magnified in (D1). Note that SGs are found only at the base of the intercellular bridge ((D1), yellow arrows). The asterisk in (D1) indicates the microtubule overlap region of the midbody. N, nucleus. Scale bars, (A)–(D), 5 μm, (D1), 1 μm.
