A Micro-RNA Connection in -Mediated Premature Senescence of Human Melanocytes
Figure 1
Melanocytes were infected with control retroviral empty vector (EVC) or with a vector expressing . Four days after infection, cells were harvested for (a) immunoblotting with specific Abs as indicated or (d) total RNA. The expression levels of the indicated senescence markers in expressing or control cells were quantified by qRT-PCR and normalized to the level of b-actin. The values represent the means and standard deviation from three independent experiments. Cells were also isolated 4 days after infection, fixed, and stained for (b) ki-67 or (c) SA-β-Gal activity. Insets: representative fields are shown for control EVC and expressing cells stained with ki-67 Ab or positive for SA-β-Gal activity.
