A Micro-RNA Connection in 𝐁𝐑𝐚𝐟^{𝐕𝟔𝟎𝟎𝐄}-Mediated Premature Senescence of Human Melanocytes

Figure 3

Melanocytes were infected with EVC or with vector expressing the indicated miRNA. (a) Quantification of miRNAs expression by qRT-PCR in indicated miRNA expressing cells. (b)–(e) The expression levels of the indicated senescence markers in the indicated miRNA expressing cells were quantified by qRT-PCR and normalized to the level of b-actin. The values represent the means and standard deviation from three independent experiments. Cells infected with the indicated miRNA expressing virus were also isolated after selection, fixed, and stained for (f) ki-67 or (g) SA-β-Gal activity.