Fibrin and Collagen Differentially but Synergistically Regulate Sprout Angiogenesis of Human Dermal Microvascular Endothelial Cells in 3-Dimensional Matrix
Figure 6
Fibrin facilitated sprout angiogenesis of HDMEC in collagen induced by angiogenic factors combo (30 ng/mL VEGF + 25 ng/mL bFGF) at 48 hr. Angiogenic factors stimulated HDMEC to invade and migrate into pure collagen gel individually without forming capillary sprouts (a). With the addition of 20% fibrin fibrils, by volume (b), or 30% fibrin fibrils, by volume (c), to collagen gel in the presence of angiogenic factors, sprout angiogenesis occurred together with individual cell invasion and migration. Angiogenic factors induced sprout angiogenesis of HDMEC in pure fibrin gel (d) (100x magnification).
