Fibrin and Collagen Differentially but Synergistically Regulate Sprout Angiogenesis of Human Dermal Microvascular Endothelial Cells in 3-Dimensional Matrix
Figure 8
Integrin alpha v beta3 antagonist Echistatin and integrin alpha 2 beta 1 antagonist ECL12 differentially but synergistically inhibit sprout angiogenesis of HDMEC in fibrin. VEGF (30 ng/mL) and bFGF (25 ng/mL) induced sprout angiogenesis in fibrin (a). Echistatin at very low dose (0.1 microgram/mL) had mild inhibitory effect on sprout angiogenesis of HDMEC in fibrin (b). While disintegrin ECL12 had no inhibitory effect on sprout angiogenesis in vitro (c), it synergistically enhanced the inhibition of sprout angiogenesis by Echistatin (d).
