Conserved Cys residues in members of the prolamin superfamily. Amino acid sequences in regions A, B, and C of Zea mays (Zm) and Oryza sativa (Os) storage proteins are compared to Helianthus annuus (Ha) SFA-8. Amino acid sequences of SFA-8 (CAA40015), 16-kD γ-zein (NP_001105337), 27-kD γ-zein (NP_001105354), 15-kD β-zein (NP_001106004), α-globulin (BAA09308), and Cys-rich 10-kD prolamin (crP10; NP_001051380) were aligned by means of CLUSTALW. For comparison, the corresponding sequence of Cys-depleted 13-kD prolamin (cpP13; NP_001055213) is shown. Two disulfide bonds in SFA-8, Cys62–Cys89 and Cys90–Cys132, are indicated with solid lines. Red-on-black letters indicate Cys residues; white-on-black letters indicate amino acid residues conserved in at least four of the sequences analyzed.
