Receptor-Mediated Endocytosis and Brain Delivery of Therapeutic Biologics

Figure 1

The cTfRMAb-ScFv fusion protein is formed by fusion of the variable region of the heavy chain (VH) of the rat 8D3 MAb against the mouse transferrin receptor (mTfR) (yellow) to the amino terminus of mouse IgG1 constant (C) region (green), and fusion of a single chain Fv (ScFv) antibody against the A amyloid peptide to the carboxyl terminus of the heavy chain C-region. The light chain is composed of the variable region of the light chain (VL) of the rat 8D3 MAb (light blue) and the mouse kappa light chain C-region (CL) (dark red). The heavy chain constant region is composed of 4 domains: CH1, hinge, CH2, and CH3. The CH2-CH3 interface is the binding site for the neonatal Fc receptor (FcRn). The ScFv is composed of the VH (dark blue) and the VL (light red) derived from the anti-AMAb (adapted from Figure 1, [52]).