Quantifying Changes in the Cellular Thiol-Disulfide Status during Differentiation of B Cells into Antibody-Secreting Plasma Cells

Table 1

Relative distribution of protein and glutathione sulfhydryl equivalents in resting B cells.

	Protein^a	Glutathione^b
% Thiols	95±4	91±5
% Disulfides^c	5.1±0.2	8.8±0.5
% PSSG^d	0.11±0.02	0.15±0.02

Values are given as the means ± SDM.
^aPercentage of measured SH/aa relative to total protein SH/aa.
^bPercentage of measured SH/aa relative to total glutathione SH/aa. Total glutathione equivalents are calculated by addition of the SH/aa values of [Total soluble GS], quantified from the TCA supernatant, and [GS in PSSG], quantified from the TCA pellet, according to Figure 1. GSH is calculated by subtracting [GS in GSSG] from [Total soluble GS].
^cValues are calculated as SH/aa equivalents engaged in disulfide bond formation.
^dValues are calculated as SH/aa equivalents engaged in PSSG formation.