Lithium Improves Survival of PC12 Pheochromocytoma Cells in High-Density Cultures and after Exposure to Toxic Compounds
Figure 3
Detection of TUNEL-positive cells (a) and release of LDH (b) in the culture medium of PC12 cells treated for 24 and 48 hours, respectively, with 10 μM TMT alone or in combination with 0.5 mM lithium. Lithium treatment completely reverses TMT-induced apoptosis (a); a significant inhibition of LDH release is observed in PC12 cells treated with doses of lithium ranging from 0.5 to 2 mM with respect to cultures treated with TMT alone. Averages ± SEM of three independent experiments. ANOVA with Bonferroni’s-corrected -test. *, ** versus untreated control.
