Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining
Figure 2
Effect of Allium cepa L. on cell viability on Pg LPS and E. coli LPS-induced RAW 264.7 cells. Cells were cultured in the presence of Pg LPS ((a), (b), and (f)) or E. coli LPS ((c), (d), and (g)), and AcE; untreated cells (Ctrl) were not exposed to AcE and DMSO was used for positive control (e). After 5 days of continuous exposure, cell proliferation was assessed using Alamar blue assay. Photographs shown are representative of results observed in multiple photographs taken for the following groups: Ctrl Pg LPS (a) and AcE 1000 μg/mL (b); Ctrl E. coli LPS (c) and AcE 1000 μg/mL (d). In (f) and (g) the columns represent the mean values of the results obtained of three independent experiments. There was no significance between control (Ctrl) and cells in all concentrations tested. There was significance between positive control (DMSO) and cells in all concentrations tested ANOVA-Tukey.
