Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining
Figure 3
Effect of Allium cepa L. on total protein levels of LPS-induced and apoptosis in RAW 264.7 cells. In (a) and (b), cells were cultured in the presence of Pg LPS (a) and E. coli LPS (b), and AcE; untreated cells (Ctrl) were not exposed to AcE. DMSO was used for positive control. After 5 days of continuous exposure, quantification of total protein levels was assessed using protein assays. In (c), activities of caspase-3 and caspase-7 were examined by caspase 3/7 assay. In (a), (b), and (c) columns represent the mean values of the results obtained of three independent experiments, and error bars represent the standard error from the means. There was no significance between control (Ctrl) and cells in all concentrations tested, ANOVA-Tukey.
