Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining

Figure 4

Effect of Allium cepa L. on cell morphology and cell count by DAPI on LPS-induced RAW 264.7 cells. RAW cells were cultured in the presence of Pg LPS ((a), (b), and (f)) or E. coli LPS ((c), (d), and (g)), and AcE; untreated cells (Ctrl) were not exposed to AcE. In (a) (Ctrl Pg LPS) and (b) (AcE 1000 μg/mL); (c) (Ctrl E. coli LPS) and (d) (AcE 1000 μg/mL) morphology was observed. DMSO was used for positive control (e). In (f) and (g), cell count by DAPI staining using a fluorescence microscope. Columns represent the mean values of the results obtained of three independent experiments. There was no significance between control (Ctrl) and cells in all concentrations tested, ANOVA-Tukey.