The Linker Histone H1.2 Is an Intermediate in the Apoptotic Response to Cytokine Deprivation in T-Effectors
Figure 1
LMB blocks cytokine deprivation induced apoptotic damage in T-effectors. (a)-(b) T-effectors were cultured with or without IL-7 (20 ng/ml) with the addition of vehicle control or LMB (0.003 ng/ml). Apoptotic nuclear damage (a) and Annexin-V binding (b) were scored after 8 and 15 hours, respectively. The dotted lines in (b) indicate cells continued in cytokine. The graph shows mean ± SD from 4 experiments. **. (c)–(e) T-effectors were cultured in the indicated conditions (T0: freshly activated) and 8 hours later cells were fractionated as described in Materials and Methods. Immunoblots of nuclear and cytoplasmic fractions obtained were probed for AIF, HP1α, and Cox-IV. Data are representative of three independent trials.
