The Linker Histone H1.2 Is an Intermediate in the Apoptotic Response to Cytokine Deprivation in T-Effectors
Figure 3
H1.2 immunoprecipitates with the mitochondrial outer-membrane resident Bak in T-effectors. (a)-(b) T-effectors were cultured without cytokine and 6 hours later immunoprecipitated (IP) with an antibody to Bak. Immunoblots of whole cell lysates (WCL) or the IP were probed with an antibody to H1.2 (a) or a pan H1 antibody (b) and α-Tubulin. (c) Reverse IP with an antibody to H1 in T-effectors cultured as described in (a)-(b) and the immunoblot probed for Bak. Actin is the specificity control. (d) T-effectors were cultured without cytokine for 6 hours and the cytoplasmic fraction obtained by subcellular fractionation as described in Materials and Methods was used as input for an IP with an antibody to Bak. A representative immunoblot for H1 in the complex IP is shown. α-Tubulin is the specificity control.
