CXCL12 Modulates Prostate Cancer Cell Adhesion by Altering the Levels or Activities of β1-Containing Integrins

Figure 1

(a) PC3 and DU145 cell attachments on FN, 50K, and H/120. A375 cell was used as a control; A375 is positive for both α4β1- and α5β1-mediated cell adhesion on FN. (b) The effect of anti-β1 integrin antibody on DU145 and PC3 cells attachment to FN (10 μg/mL). (c) The effect of anti-β1 and anti-α2β1 integrin antibodies (10 μg/mL) on DU145 and PC3 cell attachment to COL-I (5 μg/mL). The level of nonspecific binding, determined from these cells attachment to wells coated with BSA alone, was subtracted. Mouse and rat normal control antibodies have no impact on the levels of DU145 and PC3 cell attachment to FN and COL-I. Values shown are mean ± standard deviation of triplicate wells.

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