Extracellular Vesicles from Caveolin-Enriched Microdomains Regulate Hyaluronan-Mediated Sustained Vascular Integrity

Figure 5

The effects of isolated LMW-HA and HMW-HA-induced EV on human EC barrier function. Panel (a): HPMVEC were plated on transendothelial electrical resistance (TER) electrodes, grown to confluence, and switched to serum-free media and 0, 0.1, 1, or 10 μg/mL isolated LMW-HA-induced exosomes were then added. Addition of isolated LMW-HA-induced exosomes to human EC monolayers promoted barrier disruption in a dose-dependent manner. Panel (b): HPMVEC were plated on transendothelial electrical resistance (TER) electrodes, grown to confluence, and switched to serum-free media and 0, 0.1, 1, or 10 μg/mL isolated HMW-HA-induced enlargeosomes were then added. Addition of isolated HMW-HA-induced enlargeosomes to human EC monolayers induces barrier enhancement in a dose-dependent manner.

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