Impact of Hybrid and Complex N-Glycans on Cell Surface Targeting of the Endogenous Chloride Cotransporter Slc12a2

Figure 6

NKCC1 localizes in Rab11-positive compartments in COS7 cells. ((a)-(b)) Shown are representative images of COS7 cells expressing immunoreactive NKCC1 (a) or Rab11 (b). (c) (a) + (b) images superimposed to obtain an overlay image where colocalization could be estimated as yellow pixels (red + green). The scale bar represents 10 μm. (d) Semiquantitation of red (NKCC1), green (Rab11), and yellow (NKCC1 + Rab11) pixels. Shown are results obtained from at least 10 cells and represented as mean fluorescence intensity in arbitrary units ± SEM.

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