HARE-Mediated Endocytosis of Hyaluronan and Heparin Is Targeted by Different Subsets of Three Endocytic Motifs

Figure 4

HARE mediates the endocytosis of HA or Hep in the presence of M3 alone, but not in the presence of M2 or M4 alone. Cells expressing WT HARE (●), EV (○), or single-motif containing HARE CD variants +M2 (▼), +M3 (Δ in (a); ▽ in (b)), or M4 (□) were grown and treated as in Figure 2, incubated at 37°C for the indicated times with ¹²⁵I-labeled Hep (a) or HA (b), and processed to quantify uptake as described in Methods section. Values are means ± SE () and all linear regression lines had correlation coefficients ≥0.97. The three symbols for EV, +M2, and +M4 cells overlap at essentially identical positions in the bottom lines of each panel.

(a)

(b)