HARE-Mediated Endocytosis of Hyaluronan and Heparin Is Targeted by Different Subsets of Three Endocytic Motifs

Figure 6

Endocytosis of HA and Hep by HARE CD variants is blocked by a dynamin inhibitor. WT cells were washed and preincubated in Endocytosis Medium as in Figure 2 and pretreated in medium with DMSO alone (black) or with 300 μM dynasore (white) at 37°C for 30 min. The medium was then replaced with fresh media containing DMSO alone or dynasore and ¹²⁵I-labelled HA (a) or Hep (b). The cells were incubated at 37°C for 4 h and specific cell-associated ligand was determined as noted in Methods section. Values are the means ± SE () and significant differences (Student’s -test) between treated and control samples are indicated: ; .

(a)

(b)