Research Article
Effects of Activating Mutations on EGFR Cellular Protein Turnover and Amino Acid Recycling Determined Using SILAC Mass Spectrometry
Table 1
Cell lines examined and EGFR turnover rate ().
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
One wild-type and three mutant EGFR cell lines were analyzed. Cell lines with wild-type EGFR (A431), EGFR with a single activating mutant Del 746–750 (PC-9) or L858R (H3255), and the drug-resistant double mutant L858R/T790M (H1975) EGFR had turnover rate half-lives from 28 to 7.5 hours. These rates were not adjusted for amino acid recycling effects. The EGFR turnover rate half-lives in PC-9 cells dosed with inhibitors compound A and dacomitinib were reported as a range to include impact of cell viability on the measurement. |